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Index > Protein center > IGF2(Gene name) > Bovine
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  • IGF2 (Gene name),
  • Insulin-like growth factor II (Protein name ),  IGF2_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    IGF2;
    Protein name:
    Insulin-like growth factor II(IGF-II);
    Alternative:
    Erythrotropin;
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    179
    Sequence:
    50:
    MGITAGKSVL | VLLAFLAFAS | CCYAAYRPSE | TLCGGELVDT | LQFVCGDRGF | 
    100:
    YFSRPSSRIN | RRSRGIVEEC | CFRSCDLALL | ETYCATPAKS | ERDVSASTTV | 
    150:
    LPDDVTAYPV | GKFFQYDIWK | QSTQRLRRGL | PAFLRARRGR | TLAKELEALR | 
    179:
    EAKSHRPLIA | LPTQDPATHG | GASSKASSD
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    UniGene:
    UniGene:
    String:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine IGF-II
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    E0051r
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    ELISA Kit for Bovine IGF-II
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    E0051p
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    ELISA Kit for Bovine IGF-II
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    E0051m
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    ELISA Kit for Bovine IGF-II
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    ELISA Kit for Bovine IGF-II
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    E0051c
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    ELISA Kit for Bovine IGF-II
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    E0051b
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    CLIA Kit for Bovine IGF-II
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    U0051b
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    CLIA Kit for Bovine IGF-II
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    CLIA Kit for Bovine IGF-II
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    U0051h
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    CLIA Kit for Bovine IGF-II
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    96T
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    CLIA Kit for Bovine IGF-II
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    Packing:
    96T
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    CLIA Kit for Bovine IGF-II
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    U0051r
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    Polyclonal Antibody for Bovine IGF-II
    Cat.:
    P0051Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine IGF-II
    Polyclonal Antibody for Bovine IGF-II
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    P0051Rb-r
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    Polyclonal Antibody for Bovine IGF-II
    Polyclonal Antibody for Bovine IGF-II
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    P0051Rb-h
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    Polyclonal Antibody for Bovine IGF-II
    Monoclonal Antibody for Bovine IGF-II
    Monoclonal Antibody for Bovine IGF-II
    Monoclonal Antibody for Bovine IGF-II
    Monoclonal Antibody for Bovine IGF-II
    Monoclonal Antibody for Bovine IGF-II
    Monoclonal Antibody for Bovine IGF-II
    Protein for Bovine IGF-II
    Protein for Bovine IGF-II
    Protein for Bovine IGF-II
    Protein for Bovine IGF-II
    Protein for Bovine IGF-II
    Protein for Bovine IGF-II

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.7%

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    Recovery
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    Recovery was determined by spiking various levels of IGF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    91

    87-95

    Plasma

    92

    90-97

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-96%

    85-92%

    87-98%

    91-97%

    EDTA plasma(n=5)

    93-95%

    88-96%

    95-100%

    86-93%

    heparin plasma(n=5)

    88-94%

     

    91-98%

    100-106%

    80-87

     

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    References
    1. 1.
      "Promoter-specific expression of the imprinted IGF2 gene in cattle (Bos taurus)."
      Curchoe C. , Zhang S. , Bin Y. , Zhang X. , Yang L. , Feng D. , O'Neill M. , Tian X.C.
      Biol. Reprod.73:1275-1281(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      NIH - Mammalian Gene Collection (MGC) project
      Submitted (2006-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: Hereford.
      tissue: Ascending colon.
    3. 3.
      "Insulin-like growth factors I and II in fetal and adult bovine serum. Purification, primary structures, and immunological cross-reactivities."
      Honegger A. , Humbel R.E.
      J. Biol. Chem.261:569-575(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-91
    4. 4.
      "Insulin-like growth factors 1 and 2 in bovine colostrum. Sequences and biological activities compared with those of a potent truncated form."
      Francis G.L. , Upton F.M. , Ballard F.J. , McNeil K.A. , Wallace J.C.
      Biochem. J.251:95-103(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION
    5. 5.
      "A heparin-binding erythroid cell stimulating factor from fetal bovine serum has the N-terminal sequence of insulin-like growth factor II."
      Li Q. , Blacher R. , Esch F. , Congote L.F.
      Biochem. Biophys. Res. Commun.166:557-561(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-55
    6. 6.
      "The nucleotide and deduced amino acid sequences of insulin-like growth factor II cDNAs from adult bovine and fetal sheep liver."
      Brown W.M. , Dziegielewska K.M. , Foreman R.C. , Saunders N.R.
      Nucleic Acids Res.18:4614-4614(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-179
      tissue: Liver.
    7. 7.
      "Nucleotide sequence of the central coding region of bovine erythrotropin/insulin-like growth factor II cDNA from fetal intestine and northern analysis of the major IGF II transcripts at the time of hepatic erythropoiesis."
      Congote L.F. , Mazza L. , Palfree R.G.E.
      Comp. Biochem. Physiol.103B:127-131(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-86
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